This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: CACNA1E, Human
PrimePCR™ Template for SYBR® Green Assay: CACNA1E, Human
Voltage-dependent calcium channels are multisubunit complexes consisting of alpha-1 alpha-2 beta and delta subunits in a 1:1:1:1 ratio. These channels mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes including muscle contraction hormone or neurotransmitter release gene expression cell motility cell division and cell death. This gene encodes the alpha-1E subunit of the R-type calcium channels which belong to the 'high-voltage activated' group that maybe involved in the modulation of firing patterns of neurons important for information processing. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq Apr 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.