PrimePCR™ SYBR® Green Assay: HOOK3, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0029677

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0015053
Assay Design:   Intron-spanning
Chromosome Location:   8:42852735-42863049question
Amplicon Length:   111
Splice Variants Targeted:   ENST00000307602 ENST00000533539

Gene Information

Hook proteins are cytosolic coiled-coil proteins that contain conserved N-terminal domains which attach to microtubules and more divergent C-terminal domains which mediate binding to organelles. The Drosophila Hook protein is a component of the endocytic compartment.[supplied by OMIM Apr 2004]

Gene Symbol:   HOOK3
Gene Name:   hook homolog 3 (Drosophila)
Aliases:   FLJ31058, HK3
RefSeq:   NC_000008.10 NT_167187.1
Ensembl:   ENSG00000168172
Entrez:   84376
UniGene:   Hs.162852
Chromosome Mapping:   8p11.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.880000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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