This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0029317
PrimePCR™ PreAmp for SYBR® Green Assay: SLC16A3, Human
PrimePCR™ Template for SYBR® Green Assay: SLC16A3, Human
Lactic acid and pyruvate transport across plasma membranes is catalyzed by members of the proton-linked monocarboxylate transporter (MCT) family which has been designated solute carrier family-16. Each MCT appears to have slightly different substrate and inhibitor specificities and transport kinetics which are related to the metabolic requirements of the tissues in which it is found. The MCTs which include MCT1 (SLC16A1; MIM 600682) and MCT2 (SLC16A7; MIM 603654) are characterized by 12 predicted transmembrane domains (Price et al. 1998 [PubMed 9425115]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.