PrimePCR™ SYBR® Green Assay: IRF5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028441

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012423
Assay Design:   Intron-spanning
Chromosome Location:   7:128582302-128586040question
Amplicon Length:   141
Splice Variants Targeted:   ENST00000489702 ENST00000357234 ENST00000477535 ENST00000479582 ENST00000464557 ENST00000402030 ENST00000473745 ENST00000249375 ENST00000412326 ENST00000430204 ENST00000453794

Gene Information

This gene encodes a member of the interferon regulatory factor (IRF) family a group of transcription factors with diverse roles including virus-mediated activation of interferon and modulation of cell growth differentiation apoptosis and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Multiple transcript variants encoding different isoforms have been found for this gene and a 30-nt indel polymorphism (SNP rs60344245) can result in loss of a 10-aa segment. [provided by RefSeq Mar 2010]

Gene Symbol:   IRF5
Gene Name:   interferon regulatory factor 5
Aliases:   SLEB10
RefSeq:   NC_000007.13 NT_007933.15 NG_012306.1
Ensembl:   ENSG00000128604
Entrez:   3663
UniGene:   Hs.521181
Chromosome Mapping:   7q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.120000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download