PrimePCR™ SYBR® Green Assay: PLEKHG5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PLEKHG5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PLEKHG5, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012266
Assay Design:   Intron-spanning
Chromosome Location:   1:6529685-6530403question
Amplicon Length:   130
Splice Variants Targeted:   ENST00000377748 ENST00000400915 ENST00000377740 ENST00000377732 ENST00000377728 ENST00000377725 ENST00000537245 ENST00000535355 ENST00000340850 ENST00000400913 ENST00000377737 ENST00000535966 ENST00000544978

Gene Information

This gene encodes a protein that activates the nuclear factor kappa B (NFKB1) signaling pathway. Multations in this gene have been found in a family with distal spinal muscular atrophy. [provided by RefSeq Jul 2008]

Gene Symbol:   PLEKHG5
Gene Name:   pleckstrin homology domain containing, family G (with RhoGef domain) member 5
Aliases:   DSMA4, GEF720, KIAA0720
RefSeq:   NC_000001.10 NT_021937.19 NG_007978.1
Ensembl:   ENSG00000171680
Entrez:   57449
Chromosome Mapping:   1p36.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.995600
y-intercept 35.720000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download