PrimePCR™ SYBR® Green Assay: ERV3-1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: ERV3-1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ERV3-1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0011683
Assay Design:   Intron-spanning
Chromosome Location:   7:64453752-64467031question
Amplicon Length:   124
Splice Variants Targeted:   ENST00000394323

Gene Information

The human genome includes many retroelements including the human endogenous retroviruses (HERVs). ERV3 one of the most studied HERVs is thought to have integrated 30 to 40 million years ago and is present in higher primates with the exception of gorillas. Taken together the observation of genome conservation the detection of transcript expression and the presence of conserved ORFs is circumstantial evidence for a functional role. A functional role is also suggested by the observation that downregulation of ERV3 is reported in choriocarcinoma. [provided by RefSeq Jul 2008]

Gene Symbol:   ERV3-1
Gene Name:   endogenous retrovirus group 3, member 1
Aliases:   ERV-R, ERV3, ERVR, FLJ23884, HERV-R, HERVR, envR
RefSeq:   NC_000007.13 NG_006928.2 NT_007933.15
Ensembl:   ENSG00000213462
Entrez:   2086
Chromosome Mapping:   7p12-q11,7q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.240000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download