This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: ERV3-1, Human
PrimePCR™ Template for SYBR® Green Assay: ERV3-1, Human
The human genome includes many retroelements including the human endogenous retroviruses (HERVs). ERV3 one of the most studied HERVs is thought to have integrated 30 to 40 million years ago and is present in higher primates with the exception of gorillas. Taken together the observation of genome conservation the detection of transcript expression and the presence of conserved ORFs is circumstantial evidence for a functional role. A functional role is also suggested by the observation that downregulation of ERV3 is reported in choriocarcinoma. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.