This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0027984
PrimePCR™ PreAmp for SYBR® Green Assay: PAPSS2, Human
PrimePCR™ Template for SYBR® Green Assay: PAPSS2, Human
Sulfation is a common modification of endogenous (lipids proteins and carbohydrates) and exogenous (xenobiotics and drugs) compounds. In mammals the sulfate source is 3'-phosphoadenosine 5'-phosphosulfate (PAPS) created from ATP and inorganic sulfate. Two different tissue isoforms encoded by different genes synthesize PAPS. This gene encodes one of the two PAPS synthetases. Defects in this gene cause the Pakistani type of spondyloepimetaphyseal dysplasia. Two alternatively spliced transcript variants that encode different isoforms have been described for this gene. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.