PrimePCR™ SYBR® Green Assay: PDE7A, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010966
Assay Design:   Intron-spanning
Chromosome Location:   8:66659999-66695044question
Amplicon Length:   121
Splice Variants Targeted:   ENST00000401827 ENST00000379419 ENST00000523253 ENST00000396642

Gene Information

The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family and PDE7 subfamily. This PDE hydrolyzes the second messenger cAMP which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus by regulating the cellular concentration of cAMP this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq Jul 2011]

Gene Symbol:   PDE7A
Gene Name:   phosphodiesterase 7A
Aliases:   HCP1, PDE7
RefSeq:   NC_000008.10 NT_008183.19
Ensembl:   ENSG00000205268
Entrez:   5150
UniGene:   Hs.527119
Chromosome Mapping:   8q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 34.990000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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