PrimePCR™ SYBR® Green Assay: IKZF4, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009884
Assay Design:   Intron-spanning
Chromosome Location:   12:56418886-56420623question
Amplicon Length:   86
Splice Variants Targeted:   ENST00000547167 ENST00000547791 ENST00000431367 ENST00000262032

Gene Information

Members of the Ikaros (ZNFN1A1; MIM 603023) family of transcription factors which includes Eos are expressed in lymphocytes and are implicated in the control of lymphoid development.[supplied by OMIM Jul 2002]

Gene Symbol:   IKZF4
Gene Name:   IKAROS family zinc finger 4 (Eos)
Aliases:   EOS, KIAA1782, ZNFN1A4
RefSeq:   NC_000012.11 NG_023201.1 NT_029419.12
Ensembl:   ENSG00000123411
Entrez:   64375
UniGene:   Hs.553221
Chromosome Mapping:   12q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.420000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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