PrimePCR™ SYBR® Green Assay: DOCK4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027069

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009157
Assay Design:   Intron-spanning
Chromosome Location:   7:111405262-111409640question
Amplicon Length:   130
Splice Variants Targeted:   ENST00000423057 ENST00000428084 ENST00000437633 ENST00000445943 ENST00000437129 ENST00000450156 ENST00000352877 ENST00000342288 ENST00000494651

Gene Information

This gene is a member of the dedicator of cytokinesis (DOCK) family and encodes a protein with a DHR-1 (CZH-1) domain a DHR-2 (CZH-2) domain and an SH3 domain. This membrane-associated cytoplasmic protein functions as a guanine nucleotide exchange factor and is involved in regulation of adherens junctions between cells. Mutations in this gene have been associated with ovarian prostate glioma and colorectal cancers. Alternatively spliced variants which encode different protein isoforms have been described but only one has been fully characterized. [provided by RefSeq Jul 2008]

Gene Symbol:   DOCK4
Gene Name:   dedicator of cytokinesis 4
Aliases:   FLJ34238, KIAA0716, MGC134911, MGC134912
RefSeq:   NC_000007.13 NG_028060.1 NT_007933.15
Ensembl:   ENSG00000128512
Entrez:   9732
UniGene:   Hs.654652
Chromosome Mapping:   7q31.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.970000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download