PrimePCR™ SYBR® Green Assay: HGSNAT, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: HGSNAT, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: HGSNAT, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0008891
Assay Design:   Intron-spanning
Chromosome Location:   8:43037349-43046652question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000379644 ENST00000522082 ENST00000524016 ENST00000521576 ENST00000458501 ENST00000297798

Gene Information

This gene encodes a lysosomal acetyltransferase which is one of several enzymes involved in the lysosomal degradation of heparin sulfate. Mutations in this gene are associated with Sanfilippo syndrome C one type of the lysosomal storage disease mucopolysaccaridosis III which results from impaired degradation of heparan sulfate. [provided by RefSeq Jan 2009]

Gene Symbol:   HGSNAT
Gene Name:   heparan-alpha-glucosaminide N-acetyltransferase
Aliases:   DKFZp686G24175, FLJ22242, FLJ32731, HGNAT, MPS3C, TMEM76
RefSeq:   NC_000008.10 NG_009552.1 NT_167187.1
Ensembl:   ENSG00000165102
Entrez:   138050
Chromosome Mapping:   8p11.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 34.540000
Efficiency 107

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download