PrimePCR™ SYBR® Green Assay: GALNS, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0026557

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0007640
Assay Design:   Intron-spanning
Chromosome Location:   16:88904106-88907443question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000268695 ENST00000542788 ENST00000439266

Gene Information

This gene encodes N-acetylgalactosamine-6-sulfatase which is a lysosomal exohydrolase required for the degradation of the glycosaminoglycans keratan sulfate and chondroitin 6-sulfate. Sequence alterations including point missense and nonsense mutations as well as those that affect splicing result in a deficiency of this enzyme. Deficiencies of this enzyme lead to Morquio A syndrome a lysosomal storage disorder. [provided by RefSeq Jul 2008]

Gene Symbol:   GALNS
Gene Name:   galactosamine (N-acetyl)-6-sulfate sulfatase
Aliases:   FLJ17434, FLJ42844, FLJ98217, GALNAC6S, GAS, MPS4A
RefSeq:   NC_000016.9 NG_008013.1 NG_008667.1 NT_010542.15
Ensembl:   ENSG00000141012
Entrez:   2588
UniGene:   Hs.271383
Chromosome Mapping:   16q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.780000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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