This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: MGAT4A, Human
PrimePCR™ Template for SYBR® Green Assay: MGAT4A, Human
This gene encodes a key glycosyltransferase that regulates the formation of tri- and multiantennary branching structures in the Golgi apparatus. The encoded protein in addition to the related isoenzyme B catalyzes the transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc in a beta-14 linkage to the Man-alpha-13-Man-beta-14-GlcNAc arm of R-Man-alpha-16(GlcNAc-beta-12-Man-alpha-13)Man-beta-14-GlcNAc-beta-14-GlcNAc-beta-1-Asn. The encoded protein may play a role in regulating the availability of serum glycoproteins oncogenesis and differentiation. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.