This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: SLC15A2, Human
PrimePCR™ Template for SYBR® Green Assay: SLC15A2, Human
The mammalian kidney expresses a proton-coupled peptide transporter that is responsible for the absorption of small peptides as well as beta-lactam antibiotics and other peptide-like drugs from the tubular filtrate. This transporter SLC15A2 belongs to the same gene family as SLC15A1 (MIM 600544) the proton-coupled peptide transporter found in the small intestine (Liu et al 1995 [PubMed 7756356]).[supplied by OMIM Feb 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.