PrimePCR™ SYBR® Green Assay: STT3B, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0007246
Assay Design:   Intron-spanning
Chromosome Location:   3:31638315-31656623question
Amplicon Length:   138
Splice Variants Targeted:   ENST00000295770

Gene Information

The SIMP protein contains a highly immunogenic minor histocompatibility antigen epitope of 9 amino acids B6(dom1). Like ITM1 (MIM 601134) SIMP is homologous to yeast STT3 an oligosaccharyltransferase essential for cell proliferation (McBride et al. 2002 [PubMed 12439619]).[supplied by OMIM Mar 2008]

Gene Symbol:   STT3B
Gene Name:   STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae)
Aliases:   FLJ90106, SIMP, STT3-B
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000163527
Entrez:   201595
UniGene:   Hs.475812
Chromosome Mapping:   3p23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 34.960000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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