This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0026354
PrimePCR™ PreAmp for SYBR® Green Assay: RCBTB1, Human
PrimePCR™ Template for SYBR® Green Assay: RCBTB1, Human
This gene encodes a protein with an N-terminal RCC1 domain and a C-terminal BTB (broad complex tramtrack and bric-a-brac) domain. In rat over-expression of this gene in vascular smooth muscle cells induced cellular hypertrophy. In rat the C-terminus of RCBTB1 interacts with the angiotensin II receptor-1A. In humans this gene maps to a region of chromosome 13q that is frequently deleted in B-cell chronic lymphocytic leukemia and other lymphoid malignancies. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.