PrimePCR™ SYBR® Green Assay: ELAVL2, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0026344

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0007088
Assay Design:   Intron-spanning
Chromosome Location:   9:23701521-23704975question
Amplicon Length:   112
Splice Variants Targeted:   ENST00000223951 ENST00000380117 ENST00000359598 ENST00000423281 ENST00000440102 ENST00000397312 ENST00000544538 ENST00000380110

Gene Information

The protein encoded by this gene is a neural-specific RNA-binding protein that is known to bind to several 3' UTRs including its own and also that of FOS and ID. The encoded protein may recognize a GAAA motif in the RNA. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq Jan 2010]

Gene Symbol:   ELAVL2
Gene Name:   ELAV (embryonic lethal, abnormal vision, Drosophila)-like 2 (Hu antigen B)
Aliases:   HEL-N1, HELN1, HUB
RefSeq:   NC_000009.11 NT_008413.18 NG_016425.1
Ensembl:   ENSG00000107105
Entrez:   1993
UniGene:   Hs.166109
Chromosome Mapping:   9p21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997900
y-intercept 35.820000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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