PrimePCR™ SYBR® Green Assay: ABLIM3, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0007055
Assay Design:   Intron-spanning
Chromosome Location:   5:148627385-148630034question
Amplicon Length:   133
Splice Variants Targeted:   ENST00000326685 ENST00000309868 ENST00000506113 ENST00000504238 ENST00000508983 ENST00000517451 ENST00000356541 ENST00000536903

Gene Information

The LIM domain is a double zinc finger structure that promotes protein-protein interactions. LIM domain proteins such as ABLIM3 play roles in embryonic development cell lineage determination and cancer (Krupp et al. 2006 [PubMed 16328021]).[supplied by OMIM Mar 2008]

Gene Symbol:   ABLIM3
Gene Name:   actin binding LIM protein family, member 3
Aliases:   Not Available
RefSeq:   NC_000005.9 NT_029289.11
Ensembl:   ENSG00000173210
Entrez:   22885
UniGene:   Hs.49688
Chromosome Mapping:   5q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.240000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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