This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0026207
PrimePCR™ PreAmp for SYBR® Green Assay: GRIPAP1, Human
PrimePCR™ Template for SYBR® Green Assay: GRIPAP1, Human
This gene encodes a guanine nucleotide exchange factor for the Ras family of small G proteins (RasGEF). In brain studies the encoded protein was found with the GRIP/AMPA receptor complex. Multiple alternatively spliced transcript variants have been described that encode different protein isoforms; however the full-length nature and biological validity of all of these variants have not been determined.[provided by RefSeq Nov 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.