This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: SLC10A1, Human
PrimePCR™ Template for SYBR® Green Assay: SLC10A1, Human
The protein encoded by this gene belongs to the sodium/bile acid cotransporter family which are integral membrane glycoproteins that participate in the enterohepatic circulation of bile acids. Two homologous transporters are involved in the reabsorption of bile acids; the ileal sodium/bile acid cotransporter with an apical cell localization that absorbs bile acids from the intestinal lumen bile duct and kidney and the liver-specific sodium/bile acid cotransporter represented by this protein that is found in the basolateral membranes of hepatocytes. Bile acids are the catabolic product of cholesterol metabolism hence this protein is important for cholesterol homeostasis. [provided by RefSeq Oct 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.