PrimePCR™ SYBR® Green Assay: SLC10A1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0006478
Assay Design:   Intron-spanning
Chromosome Location:   14:70245993-70252886question
Amplicon Length:   128
Splice Variants Targeted:   ENST00000216540

Gene Information

The protein encoded by this gene belongs to the sodium/bile acid cotransporter family which are integral membrane glycoproteins that participate in the enterohepatic circulation of bile acids. Two homologous transporters are involved in the reabsorption of bile acids; the ileal sodium/bile acid cotransporter with an apical cell localization that absorbs bile acids from the intestinal lumen bile duct and kidney and the liver-specific sodium/bile acid cotransporter represented by this protein that is found in the basolateral membranes of hepatocytes. Bile acids are the catabolic product of cholesterol metabolism hence this protein is important for cholesterol homeostasis. [provided by RefSeq Oct 2011]

Gene Symbol:   SLC10A1
Gene Name:   solute carrier family 10 (sodium/bile acid cotransporter family), member 1
Aliases:   NTCP
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000100652
Entrez:   6554
UniGene:   Hs.952
Chromosome Mapping:   14q24.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.220000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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