This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0058414
PrimePCR™ PreAmp for SYBR® Green Assay: RTN1, Human
PrimePCR™ Template for SYBR® Green Assay: RTN1, Human
This gene belongs to the family of reticulon encoding genes. Reticulons are associated with the endoplasmic reticulum and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. This gene is considered to be a specific marker for neurological diseases and cancer and is a potential molecular target for therapy. Alternative splicing results in multiple transcript variants. [provided by RefSeq Jul 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.