PrimePCR™ SYBR® Green Assay: AMT, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0058373

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: AMT, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: AMT, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0057268
Assay Design:   exonic
Chromosome Location:   3:49456487-49456584question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000427987 ENST00000458307 ENST00000273588 ENST00000430521 ENST00000395338 ENST00000538581 ENST00000546031 ENST00000260950 ENST00000426034 ENST00000441239 ENST00000441627 ENST00000265346 ENST00000433667 ENST00000309315 ENST00000413916

Gene Information

This gene encodes one of four critical components of the glycine cleavage system. Mutations in this gene have been associated with glycine encephalopathy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Sep 2011]

Gene Symbol:   AMT
Gene Name:   aminomethyltransferase
Aliases:   GCE, GCST, GCVT, NKH
RefSeq:   NC_000003.11 NG_015986.1 NT_022517.18
Ensembl:   ENSG00000145020
Entrez:   275
Chromosome Mapping:   3p21.2-p21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.130000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download