PrimePCR™ SYBR® Green Assay: RPH3AL, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0057862

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056757
Assay Design:   exonic
Chromosome Location:   17:63470-63561question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000291187 ENST00000588931 ENST00000320301 ENST00000395430 ENST00000395432 ENST00000541194 ENST00000537928 ENST00000427531 ENST00000544394 ENST00000262961 ENST00000331302 ENST00000323434 ENST00000536489 ENST00000278379 ENST00000395750 ENST00000395753 ENST00000574223 ENST00000596450 ENST00000594863 ENST00000597943

Gene Information

The protein encoded by this gene plays a direct regulatory role in calcium-ion-dependent exocytosis in both endocrine and exocrine cells and plays a key role in insulin secretion by pancreatic cells. This gene is likely a tumor suppressor. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq Jun 2010]

Gene Symbol:   RPH3AL
Gene Name:   rabphilin 3A-like (without C2 domains)
Aliases:   NOC2
RefSeq:   NC_000017.10 NT_024972.8 NW_003315952.1 NG_023031.1
Ensembl:   ENSG00000181031
Entrez:   9501
UniGene:   Hs.651925
Chromosome Mapping:   17p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.980000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download