PrimePCR™ SYBR® Green Assay: LILRA6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0056067

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: LILRA6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: LILRA6, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048807
Assay Design:   exonic
Chromosome Location:   19:54745972-54746082question
Amplicon Length:   81
Splice Variants Targeted:   ENST00000391750 ENST00000346401 ENST00000245620 ENST00000445347 ENST00000407860 ENST00000424807 ENST00000396365 ENST00000245621 ENST00000440558 ENST00000270464 ENST00000419410 ENST00000391735 ENST00000301219 ENST00000346508 ENST00000446712 ENST00000432233 ENST00000391736 ENST00000430952 ENST00000391734 ENST00000391733 ENST00000434286 ENST00000270452 ENST00000575959 ENST00000571923 ENST00000573711 ENST00000575016 ENST00000575713 ENST00000573666 ENST00000576712 ENST00000576133 ENST00000572093 ENST

Gene Information

Description Not Available

Gene Symbol:   LILRA6
Gene Name:   leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 6
Aliases:   CD85b, ILT5, ILT8, LILRB3, LILRB6
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000244482
Entrez:   79168
Chromosome Mapping:   19q13.4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.600000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download