PrimePCR™ SYBR® Green Assay: ZNF544, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0055540

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ZNF544, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZNF544, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048280
Assay Design:   exonic
Chromosome Location:   19:58755416-58757749question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000573833 ENST00000224600 ENST00000318003 ENST00000370223 ENST00000370220 ENST00000407006 ENST00000439238 ENST00000338820 ENST00000537645 ENST00000360338 ENST00000599602 ENST00000321545 ENST00000443917 ENST00000598744 ENST00000282286 ENST00000391705 ENST00000299687 ENST00000354670 ENST00000397573 ENST00000220446 ENST00000306376 ENST00000434744 ENST00000367433 ENST00000294725 ENST00000367431 ENST00000599227 ENST00000597240 ENST00000594384 ENST00000596825 ENST00000596652 ENST00000600044 ENST

Gene Information

Description Not Available

Gene Symbol:   ZNF544
Gene Name:   zinc finger protein 544
Aliases:   Not Available
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000198131
Entrez:   27300
Chromosome Mapping:   19q13.43

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 36.540000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download