PrimePCR™ SYBR® Green Assay: SLC25A29, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0055416

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SLC25A29, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SLC25A29, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048156
Assay Design:   exonic
Chromosome Location:   14:100758797-100758927question
Amplicon Length:   101
Splice Variants Targeted:   ENST00000549629 ENST00000400023 ENST00000359232 ENST00000554912 ENST00000392908 ENST00000556505 ENST00000555927 ENST00000539621 ENST00000250237 ENST00000377346 ENST00000361110 ENST00000536656 ENST00000543390 ENST00000271620 ENST00000431193 ENST00000368936

Gene Information

Description Not Available

Gene Symbol:   SLC25A29
Gene Name:   solute carrier family 25, member 29
Aliases:   C14orf69, CACL, FLJ38975
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000197119
Entrez:   123096
Chromosome Mapping:   14q32.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.240000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download