PrimePCR™ SYBR® Green Assay: NRN1L, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0055210

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: NRN1L, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NRN1L, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047950
Assay Design:   exonic
Chromosome Location:   16:67920026-67920153question
Amplicon Length:   98
Splice Variants Targeted:   ENST00000585038 ENST00000429452 ENST00000414984 ENST00000418543 ENST00000547797 ENST00000242796 ENST00000395094 ENST00000252799 ENST00000339176 ENST00000576147 ENST00000290378 ENST00000409858 ENST00000360271 ENST00000400274 ENST00000417564 ENST00000318711 ENST00000427143 ENST00000375180 ENST00000328235 ENST00000285298 ENST00000372073 ENST00000288381 ENST00000372068 ENST00000372072 ENST00000382031 ENST00000300231 ENST00000399453 ENST00000375173 ENST00000341170 ENST00000375736 ENST00000426073 ENST

Gene Information

Description Not Available

Gene Symbol:   NRN1L
Gene Name:   neuritin 1-like
Aliases:   MGC118990, MGC118993, UNQ2446
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000188038
Entrez:   123904
Chromosome Mapping:   16q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.500000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download