PrimePCR™ SYBR® Green Assay: DNAH14, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0055033

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: DNAH14, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: DNAH14, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0047773
Assay Design:   exonic
Chromosome Location:   1:225152188-225152289question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000445597 ENST00000400952 ENST00000366849 ENST00000366848 ENST00000439375 ENST00000433124 ENST00000430092 ENST00000366850

Gene Information

Dyneins are microtubule-associated motor protein complexes composed of several heavy light and intermediate chains. Two major classes of dyneins axonemal and cytoplasmic have been identified. DNAH14 is an axonemal dynein heavy chain (DHC) (Vaughan et al. 1996 [PubMed 8812413]).[supplied by OMIM Mar 2008]

Gene Symbol:   DNAH14
Gene Name:   dynein, axonemal, heavy chain 14
Aliases:   C1orf67, DKFZp781B1548, Dnahc14, HL-18, HL18, MGC149665, MGC149666, MGC27277, MGC51214
RefSeq:   NC_000001.10 NT_167186.1
Ensembl:   ENSG00000185842
Entrez:   127602
Chromosome Mapping:   1q42.12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.910000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download