PrimePCR™ SYBR® Green Assay: ZNF440, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0054048

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ZNF440, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZNF440, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046787
Assay Design:   exonic
Chromosome Location:   19:11942283-11942403question
Amplicon Length:   91
Splice Variants Targeted:   ENST00000414255 ENST00000427505 ENST00000457526 ENST00000304060 ENST00000455282 ENST00000304030 ENST00000429654 ENST00000445911 ENST00000340180 ENST00000592625 ENST00000586494 ENST00000343949 ENST00000358987 ENST00000538752 ENST00000545530 ENST00000590798 ENST00000254321 ENST00000482090 ENST00000419886 ENST00000344980 ENST00000550507 ENST00000455504 ENST00000552904 ENST00000430298 ENST00000596883 ENST00000339302 ENST00000334213 ENST00000418866

Gene Information

Description Not Available

Gene Symbol:   ZNF440
Gene Name:   zinc finger protein 440
Aliases:   FLJ37933
RefSeq:   NC_000019.9 NT_011295.11
Ensembl:   ENSG00000171295
Entrez:   126070
Chromosome Mapping:   19p13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 36.700000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download