PrimePCR™ SYBR® Green Assay: GSTA4, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0054011

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046750
Assay Design:   exonic
Chromosome Location:   6:52847486-52849370question
Amplicon Length:   97
Splice Variants Targeted:   ENST00000370963 ENST00000370960 ENST00000370959 ENST00000457564 ENST00000541324

Gene Information

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes are involved in cellular defense against toxic carcinogenic and pharmacologically active electrophilic compounds. At present eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha kappa mu omega pi sigma theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class. The alpha class genes which are located in a cluster on chromosome 6 are highly related and encode enzymes with glutathione peroxidase activity that function in the detoxification of lipid peroxidation products. Reactive electrophiles produced by oxidative metabolism have been linked to a number of degenerative diseases including Parkinson's disease Alzheimer's disease cataract formation and atherosclerosis. [provided by RefSeq Jul 2008]

Gene Symbol:   GSTA4
Gene Name:   glutathione S-transferase alpha 4
Aliases:   DKFZp686D21185, GSTA4-4, GTA4
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000170899
Entrez:   2941
UniGene:   Hs.485557
Chromosome Mapping:   6p12.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997400
y-intercept 36.390000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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