This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0053950
PrimePCR™ PreAmp for SYBR® Green Assay: ANKK1, Human
PrimePCR™ Template for SYBR® Green Assay: ANKK1, Human
The protein encoded by this gene belongs to the Ser/Thr protein kinase family and protein kinase superfamily involved in signal transduction pathways. This gene is closely linked to DRD2 gene (GeneID:1813) on chr 11 and a well studied restriction fragment length polymorphism (RFLP) designated TaqIA was originally associated with the DRD2 gene however later was determined to be located in exon 8 of ANKK1 gene (PMIDs: 18621654 15146457) where it causes a nonconservative amino acid substitution. It is not clear if this gene plays any role in neuropsychiatric disorders previously associated with Taq1A RFLP. [provided by RefSeq Sep 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.