PrimePCR™ SYBR® Green Assay: TSPAN5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0053836

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046575
Assay Design:   exonic
Chromosome Location:   4:99407943-99428855question
Amplicon Length:   90
Splice Variants Targeted:   ENST00000305798 ENST00000505184 ENST00000515287 ENST00000511651

Gene Information

The protein encoded by this gene is a member of the transmembrane 4 superfamily also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development activation growth and motility. [provided by RefSeq Jul 2008]

Gene Symbol:   TSPAN5
Gene Name:   tetraspanin 5
Aliases:   NET-4, NET4, TM4SF9, TSPAN-5
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000168785
Entrez:   10098
UniGene:   Hs.118118
Chromosome Mapping:   4q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 36.900000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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