PrimePCR™ SYBR® Green Assay: ZNF91, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0053706

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ZNF91, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZNF91, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046445
Assay Design:   exonic
Chromosome Location:   19:23545340-23545467question
Amplicon Length:   98
Splice Variants Targeted:   ENST00000328747 ENST00000431504 ENST00000450302 ENST00000357512 ENST00000601440 ENST00000595405 ENST00000328178 ENST00000599885 ENST00000345030 ENST00000599064 ENST00000596534 ENST00000598862 ENST00000595742 ENST00000601023 ENST00000261560 ENST00000597424 ENST00000597086 ENST00000596143 ENST00000596367 ENST00000601416 ENST00000291770 ENST00000397126 ENST00000397121 ENST00000357774 ENST00000593657 ENST00000596209 ENST00000397104 ENST00000594710 ENST00000599851 ENST00000597761 ENST00000300619 ENST

Gene Information

The ZNF91 gene encodes a zinc finger protein of the KRAB (Kruppel-associated box) subfamily (Bellefroid et al. 1991 1993 [PubMed 2023909] [PubMed 8467795]).[supplied by OMIM May 2010]

Gene Symbol:   ZNF91
Gene Name:   zinc finger protein 91
Aliases:   HPF7, HTF10
RefSeq:   NC_000019.9 NT_011295.11
Ensembl:   ENSG00000167232
Entrez:   7644
Chromosome Mapping:   19p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 36.150000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download