PrimePCR™ SYBR® Green Assay: SEPT8, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best specificity; Same primer pair as used in probe assay qHsaCEP0053478

List Price:    $130.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046217
Assay Design:   exonic
Chromosome Location:   5:132099998-132100088question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000359600 ENST00000378719 ENST00000378721 ENST00000296873 ENST00000378706 ENST00000378699 ENST00000378701 ENST00000458488 ENST00000453480 ENST00000414594 ENST00000371493 ENST00000448933 ENST00000322122 ENST00000313164

Gene Information

SEPT8 is a member of the highly conserved septin family. Septins are 40- to 60-kD GTPases that assemble as filamentous scaffolds. They are involved in the organization of submembranous structures in neuronal polarity and in vesicle trafficking (Blaser et al. 2003 [PubMed 12909369]).[supplied by OMIM Mar 2008]

Gene Symbol:   SEPT8
Gene Name:   septin 8
Aliases:   KIAA0202, SEP2
RefSeq:   NC_000005.9 NT_034772.6
Ensembl:   ENSG00000164402
Entrez:   23176
UniGene:   Hs.522057
Chromosome Mapping:   5q31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.150000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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