This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0053413
PrimePCR™ PreAmp for SYBR® Green Assay: SLBP, Human
PrimePCR™ Template for SYBR® Green Assay: SLBP, Human
This gene encodes a protein that binds to the stem-loop structure in replication-dependent histone mRNAs. Histone mRNAs do not contain introns or polyadenylation signals and are processed by endonucleolytic cleavage. The stem-loop structure is essential for efficient processing but this structure also controls the transport translation and stability of histone mRNAs. Expression of the protein is regulated during the cell cycle increasing more than 10-fold during the latter part of G1. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.