PrimePCR™ SYBR® Green Assay: NPEPPS, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052263

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: NPEPPS, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NPEPPS, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045001
Assay Design:   exonic
Chromosome Location:   17:45668128-45668241question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000358894 ENST00000422202 ENST00000326996 ENST00000435874 ENST00000530173 ENST00000322157 ENST00000527964 ENST00000527360 ENST00000544660 ENST00000295878 ENST00000491000 ENST00000545063 ENST00000537432 ENST00000520237 ENST00000523089 ENST00000518004 ENST00000544906 ENST00000312412 ENST00000580223 ENST00000331427 ENST00000562825 ENST00000564952 ENST00000565045

Gene Information

This gene encodes the puromycin-sensitive aminopeptidase a zinc metallopeptidase which hydrolyzes amino acids from the N-terminus of its substrate. The protein has been localized to both the cytoplasm and to cellular membranes. This enzyme degrades enkaphalins in the brain and studies in mouse suggest that it is involved in proteolytic events regulating the cell cycle. [provided by RefSeq Jul 2008]

Gene Symbol:   NPEPPS
Gene Name:   aminopeptidase puromycin sensitive
Aliases:   AAP-S, MP100, PSA
RefSeq:   NC_000017.10 NT_010783.15 NW_003315948.1
Ensembl:   ENSG00000141279
Entrez:   9520
Chromosome Mapping:   17q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.020000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download