PrimePCR™ SYBR® Green Assay: IAPP, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051161

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: IAPP, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: IAPP, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043899
Assay Design:   exonic
Chromosome Location:   12:21531174-21531325question
Amplicon Length:   122
Splice Variants Targeted:   ENST00000539393 ENST00000240652 ENST00000542023 ENST00000537593 ENST00000535428

Gene Information

Islet or insulinoma amyloid polypeptide is commonly found in pancreatic islets of patients suffering diabetes mellitus type II or harboring an insulinoma. While the association of amylin with the development of type II diabetes has been known for some time a direct causative role for amylin has been harder to establish. Studies suggest that amylin like the related beta-amyloid (Abeta) associated with Alzheimer's disease can induce apoptotic cell-death in particular cultured cells an effect that may be relevant to the development of type II diabetes. [provided by RefSeq Apr 2011]

Gene Symbol:   IAPP
Gene Name:   islet amyloid polypeptide
Aliases:   DAP, IAP
RefSeq:   NC_000012.11 NT_009714.17
Ensembl:   ENSG00000121351
Entrez:   3375
Chromosome Mapping:   12p12.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 36.550000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download