PrimePCR™ SYBR® Green Assay: DSE, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0050670

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: DSE, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: DSE, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043408
Assay Design:   exonic
Chromosome Location:   6:116720481-116720589question
Amplicon Length:   79
Splice Variants Targeted:   ENST00000537552 ENST00000430252 ENST00000331677 ENST00000540275 ENST00000537543 ENST00000452085 ENST00000359564 ENST00000337137 ENST00000316124 ENST00000377765 ENST00000539465 ENST00000243389 ENST00000283033 ENST00000356957 ENST00000379066 ENST00000234179 ENST00000369584 ENST00000305344 ENST00000295738 ENST00000396775 ENST00000205557 ENST00000409645

Gene Information

The protein encoded by this gene is a tumor-rejection antigen. This antigen possesses tumor epitopes capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes in cancer patients and may be useful for specific immunotherapy. This gene product is localized to the endoplasmic reticulum. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   DSE
Gene Name:   dermatan sulfate epimerase
Aliases:   DSEPI, SART2
RefSeq:   NG_016217.1 NC_000006.11 NT_025741.15
Ensembl:   ENSG00000111817
Entrez:   29940
Chromosome Mapping:   6q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.450000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download