PrimePCR™ SYBR® Green Assay: CT47A6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041853

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0041784
Assay Design:   exonic
Chromosome Location:   X:120095116-120095273question
Amplicon Length:   128
Splice Variants Targeted:   ENST00000382622 ENST00000452611 ENST00000256339 ENST00000555664 ENST00000553484 ENST00000393151 ENST00000221347 ENST00000325888 ENST00000346177 ENST00000371084 ENST00000205890 ENST00000255008 ENST00000371311 ENST00000419982 ENST00000416816 ENST00000457020 ENST00000430448 ENST00000417256 ENST00000457977 ENST00000434883 ENST00000439466 ENST00000419194 ENST00000443600 ENST00000415858 ENST00000441330 ENST00000433030 ENST00000434414 ENST00000371280 ENST00000458218 ENST00000360461 ENST00000565899

Gene Information

This locus represents a member of the cancer/testis gene family 47. This family also known as CT47 is comprised of 13 nearly identical loci clustered at Xq24. [provided by RefSeq Sep 2010]

Gene Symbol:   CT47A6
Gene Name:   cancer/testis antigen family 47, member A6
Aliases:   CT47.6, CT47A1, CT47A10, CT47A11, CT47A12, CT47A2, CT47A3, CT47A4, CT47A5, CT47A7, CT47A8, CT47A9
RefSeq:   NC_000023.10 NG_027736.1 NG_027739.1 NT_011786.16
Ensembl:   ENSG00000226023
Entrez:   728062
UniGene:   Hs.723061
Chromosome Mapping:   Xq24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 34.340000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download