PrimePCR™ SYBR® Green Assay: RBM14-RBM4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041555

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RBM14-RBM4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RBM14-RBM4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038833
Assay Design:   exonic
Chromosome Location:   11:66413566-66413680question
Amplicon Length:   85
Splice Variants Targeted:   ENST00000412278 ENST00000500635 ENST00000514361 ENST00000310092 ENST00000408993 ENST00000398692 ENST00000530235 ENST00000409406 ENST00000329773

Gene Information

This locus represents naturally occurring read-through transcription between the neighboring RBM14 (RNA binding motif protein 14) and RBM4 (RNA binding motif protein 4) genes. Alternative splicing results in multiple transcript variants one of which encodes a fusion protein that shares sequence identity with each individual gene product. This fusion protein contains RRM and zinc finger domains and it functions to stimulate transcription in a hormone and receptor-dependent manner. [provided by RefSeq Nov 2010]

Gene Symbol:   RBM14-RBM4
Gene Name:   RBM14-RBM4 readthrough
Aliases:   COAZ, FLJ78260
RefSeq:   Not Available
Ensembl:   ENSG00000248643
Entrez:   100526737
Chromosome Mapping:   11q

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.290000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download