PrimePCR™ SYBR® Green Assay: CCL3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041355

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038633
Assay Design:   exonic
Chromosome Location:   17:34415896-34415987question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000442488 ENST00000427185 ENST00000331178 ENST00000226091 ENST00000261769 ENST00000422202 ENST00000429149 ENST00000336798 ENST00000394329 ENST00000225245 ENST00000378354 ENST00000379388 ENST00000422211 ENST00000272252 ENST00000225396 ENST00000566881 ENST00000406386 ENST00000565629

Gene Information

This locus represents a small inducible cytokine. The encoded protein also known as macrophage inflammatory protein 1 alpha plays a role in inflammatory responses through binding to the receptors CCR1 CCR4 and CCR5. Polymorphisms at this locus may be associated with both resistance and susceptibility to infection by human immunodeficiency virus type 1.[provided by RefSeq Sep 2010]

Gene Symbol:   CCL3
Gene Name:   chemokine (C-C motif) ligand 3
Aliases:   G0S19-1, LD78ALPHA, MIP-1-alpha, MIP1A, SCYA3
RefSeq:   NC_000017.10 NG_027730.1 NT_010799.15
Ensembl:   ENSG00000006075
Entrez:   6348
UniGene:   Hs.514107
Chromosome Mapping:   17q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.800000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download