PrimePCR™ SYBR® Green Assay: MGAT4B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041246

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038524
Assay Design:   exonic
Chromosome Location:   5:179226954-179227044question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000518778 ENST00000337755 ENST00000292591 ENST00000519836 ENST00000518980 ENST00000520875 ENST00000518867 ENST00000523108

Gene Information

This gene encodes a key glycosyltransferase that regulates the formation of tri- and multiantennary branching structures in the Golgi apparatus. The encoded protein in addition to the related isoenzyme A catalyzes the transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc in a beta-14 linkage to the Man-alpha-13-Man-beta-14-GlcNAc arm of R-Man-alpha-16(GlcNAc-beta-12-Man-alpha-13)Man-beta-14-GlcNAc-beta-14-GlcNAc-beta-1-Asn. The encoded protein may play a role in regulating the availability of serum glycoproteins oncogenesis and differentiation. [provided by RefSeq Jul 2008]

Gene Symbol:   MGAT4B
Gene Name:   mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme B
Aliases:   GNT-IV, GNT-IVB
RefSeq:   NC_000005.9 NG_011342.1 NG_028161.1 NT_023133.13
Ensembl:   ENSG00000161013
Entrez:   11282
UniGene:   Hs.567419
Chromosome Mapping:   5q35

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.470000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download