PrimePCR™ SYBR® Green Assay: KRTAP5-7, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041166

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038444
Assay Design:   exonic
Chromosome Location:   11:71238922-71239043question
Amplicon Length:   92
Splice Variants Targeted:   ENST00000399682 ENST00000529694 ENST00000257940 ENST00000269554 ENST00000398536 ENST00000409120 ENST00000496961 ENST00000310611 ENST00000420578 ENST00000430195 ENST00000377298 ENST00000435891 ENST00000361311 ENST00000377288 ENST00000361820

Gene Information

Description Not Available

Gene Symbol:   KRTAP5-7
Gene Name:   keratin associated protein 5-7
Aliases:   KRTAP5-3, KRTAP5.7
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000244411
Entrez:   440050
UniGene:   Hs.710675
Chromosome Mapping:   11q13.4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.220000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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