PrimePCR™ SYBR® Green Assay: FAM195B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041128

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038406
Assay Design:   exonic
Chromosome Location:   17:79782172-79782384question
Amplicon Length:   89
Splice Variants Targeted:   ENST00000455127 ENST00000576730 ENST00000572645 ENST00000538396 ENST00000573478 ENST00000574190 ENST00000570507 ENST00000576679 ENST00000576431 ENST00000575061 ENST00000457257 ENST00000545797 ENST00000301264 ENST00000305748

Gene Information

Description Not Available

Gene Symbol:   FAM195B
Gene Name:   family with sequence similarity 195, member B
Aliases:   DKFZp686A17165, MGC133184
RefSeq:   NC_000017.10 NT_010663.15
Ensembl:   ENSG00000225663
Entrez:   348262
UniGene:   Hs.514632
Chromosome Mapping:   17q25.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.150000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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