This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0040994
PrimePCR™ PreAmp for SYBR® Green Assay: ZNF124, Human
PrimePCR™ Template for SYBR® Green Assay: ZNF124, Human
This gene encodes a member of the Kruppel-type (C2H2) zinc finger protein family. It has a KRAB-A box at its amino terminus and seven consecutively repeated zinc finger motifs at its carboxy terminus. Expression of this gene is increased after vascular endothelial growth factor (VEGF) stimulation in human leukemia cell lines and its expression inhibits apoptotic cell death induced by irradiation or exposure to etoposide. These attributes suggest that this protein may act as a transcription factor. Alternative splicing results in multiple transcript variants encoding distinct proteins. Additional transcript variants have been described for this gene but their biological validity has not been confirmed. This gene has a pseudogene on chromosome 14. [provided by RefSeq Aug 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.