PrimePCR™ SYBR® Green Assay: CSTF3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040916

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CSTF3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CSTF3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038194
Assay Design:   exonic
Chromosome Location:   11:33182845-33182934question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000543006 ENST00000312675 ENST00000593360 ENST00000331426 ENST00000340067 ENST00000526208 ENST00000525447 ENST00000529350 ENST00000530018 ENST00000528776 ENST00000306390 ENST00000546151 ENST00000273375 ENST00000323959 ENST00000524827 ENST00000438862 ENST00000431742 ENST00000524775 ENST00000428302 ENST00000436030 ENST00000435007 ENST00000437153 ENST00000449633 ENST00000438769 ENST00000442871 ENST00000411608 ENST00000433769 ENST00000441161 ENST00000397278 ENST00000319136 ENST00000422706 ENST

Gene Information

The protein encoded by this gene is one of three (including CSTF1 and CSTF2) cleavage stimulation factors that combine to form the cleavage stimulation factor complex (CSTF). This complex is involved in the polyadenylation and 3' end cleavage of pre-mRNAs. The encoded protein functions as a homodimer and interacts directly with both CSTF1 and CSTF2 in the CSTF complex. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:   CSTF3
Gene Name:   cleavage stimulation factor, 3' pre-RNA, subunit 3, 77kDa
Aliases:   CSTF-77, MGC117398, MGC43001, MGC75122
RefSeq:   NC_000011.9 NT_009237.18
Ensembl:   ENSG00000176102
Entrez:   1479
Chromosome Mapping:   11p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.550000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download