PrimePCR™ SYBR® Green Assay: ZEB2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040872

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ZEB2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZEB2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038149
Assay Design:   exonic
Chromosome Location:   2:145274845-145274981question
Amplicon Length:   107
Splice Variants Targeted:   ENST00000546474 ENST00000303660 ENST00000409487 ENST00000539609 ENST00000558170 ENST00000427902 ENST00000392861 ENST00000409211 ENST00000435831 ENST00000444559 ENST00000298440 ENST00000543457 ENST00000332567 ENST00000262715 ENST00000322507 ENST00000509883 ENST00000514251 ENST00000441582 ENST00000321464

Gene Information

The protein encoded by this gene is a member of the Zfh1 family of 2-handed zinc finger/homeodomain proteins. It is located in the nucleus and functions as a DNA-binding transcriptional repressor that interacts with activated SMADs. Mutations in this gene are associated with Hirschsprung disease/Mowat-Wilson syndrome. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq Jan 2010]

Gene Symbol:   ZEB2
Gene Name:   zinc finger E-box binding homeobox 2
Aliases:   FLJ42816, HSPC082, KIAA0569, SIP-1, SIP1, SMADIP1, ZFHX1B
RefSeq:   NC_000002.11 NT_022135.16 NG_016431.1
Ensembl:   ENSG00000169554
Entrez:   9839
Chromosome Mapping:   2q22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.600000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download