This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0040823
PrimePCR™ PreAmp for SYBR® Green Assay: ARHGAP27, Human
PrimePCR™ Template for SYBR® Green Assay: ARHGAP27, Human
Rho (see ARHA; MIM 165390)-like small GTPases are involved in many cellular processes and they are inactive in the GDP-bound state and active in the GTP-bound state. GTPase-activating proteins such as ARHGAP27 inhibit Rho-like proteins by stimulating their intrinsic GTPase activity (Katoh and Katoh 2004 [PubMed 15492870]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.