PrimePCR™ SYBR® Green Assay: PCDH11X, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040685

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PCDH11X, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PCDH11X, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037962
Assay Design:   exonic
Chromosome Location:   X:91090528-91090652question
Amplicon Length:   95
Splice Variants Targeted:   ENST00000333703 ENST00000362095 ENST00000400457 ENST00000215473 ENST00000395337 ENST00000361724 ENST00000373094 ENST00000373097 ENST00000361655 ENST00000406881 ENST00000373088 ENST00000504220 ENST00000298274

Gene Information

This gene belongs to the protocadherin gene family a subfamily of the cadherin superfamily. The encoded protein consists of an extracellular domain containing 7 cadherin repeats a transmembrane domain and a cytoplasmic tail that differs from those of the classical cadherins. The gene is located in a major X/Y block of homology and its Y homolog despite divergence leading to coding region changes is the most closely related cadherin family member. The protein is thought to play a fundamental role in cell-cell recognition essential for the segmental development and function of the central nervous system. Transcripts arising from alternative splicing encode isoforms with variable cytoplasmic domains. [provided by RefSeq Jul 2008]

Gene Symbol:   PCDH11X
Gene Name:   protocadherin 11 X-linked
Aliases:   PCDH-X, PCDH11, PCDHX
RefSeq:   NC_000023.10 NT_011651.17 NG_016251.1
Ensembl:   ENSG00000102290
Entrez:   27328
Chromosome Mapping:   Xq21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.800000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download