PrimePCR™ SYBR® Green Assay: RP9, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040524

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RP9, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RP9, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037801
Assay Design:   exonic
Chromosome Location:   7:33134428-33134557question
Amplicon Length:   100
Splice Variants Targeted:   ENST00000297157

Gene Information

The protein encoded by this gene can be bound and phosphorylated by the protooncogene PIM1 product a serine/threonine protein kinase . This protein localizes in nuclear speckles containing the splicing factors and has a role in pre-mRNA splicing. CBF1-interacting protein (CIR) a corepressor of CBF1 can also bind to this protein and effects alternative splicing. Mutations in this gene result in autosomal dominant retinitis pigmentosa-9. This gene has a pseudogene (GeneID: 441212) which is located in tandem array approximately 166 kb distal to this gene. [provided by RefSeq Sep 2009]

Gene Symbol:   RP9
Gene Name:   retinitis pigmentosa 9 (autosomal dominant)
Aliases:   PAP-1
RefSeq:   NC_000007.13 NG_012968.1 NT_007819.17
Ensembl:   ENSG00000164610
Entrez:   6100
Chromosome Mapping:   7p14.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.230000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download